Composite

Part:BBa_K209417

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)


SSFYFP-hM4D-ActA(30-612)



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 4719
    Illegal NheI site found at 6375
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BamHI site found at 706
    Illegal BamHI site found at 5212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 3688
    Illegal PstI site found at 4483
    Illegal NgoMIV site found at 4590
    Illegal NgoMIV site found at 6243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 4993


[edit]
Categories
Parameters
None